伪狂犬病病毒感染对ST细胞生长及ATP酶活性的影响

Effect of pseudorabies virus on the cell proliferation and ATPase activity of ST cells in vitro

以伪狂犬病病毒(PRV)容A株体外感染ST细胞为模型,观察PRV对ST细胞生长状态以及胞内Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和总ATP酶活性的影响。根据ST细胞对PRV的敏感剂量,接种ST细胞后第2、12、24、36和48小时观察细胞的生长状态,MTT法检测细胞的增殖能力,用试剂盒检测细胞的ATP酶(Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和总ATP酶)活性。结果,与对照组相比,在感染后第24小时之前试验组ST细胞的生长状态较好;24h后,细胞活性降低,逐渐发生细胞凋亡。Na+-K+-ATP酶活性、Ca2+-Mg2+-ATP酶活性和总ATP酶活性在PRV感染过程中变化趋势相似,PRV感染后第24小时之前,Na+-K+-ATP酶活性、Ca2+-Mg2+-ATP酶活性和总ATP酶活性略高于对照组;第36小时,细胞ATP酶总体活性水平明显低于对照组(P<0.05);第48小时两组之间ATP酶活性差异极显著(P<0.01),其中Ca2+-Mg2+-ATP酶活性较为敏感。

In this study,we evaluated the influence of pseudorabies virus RA strain（PRV-RA）on cell proliferation and on activity of Na＋-K＋-ATPase,Ca2＋-Mg2＋-ATPase and total cell ATPase in swine testis（ST） cells.The ST cells were infected with the sensitive dose of PRV-RA,the kinetic was adopted at hour 2,12,24,36 and 48 post-inoculation,respectively.The results of MTT assays demonstrated that PRV-RA could promote cell growth during the early stages of infection,and the proliferation was inhibited after 24 h.Simultaneously,the activity of the cellular ATPase were evaluated.The activity of Na＋-K＋-ATPase,Ca2＋-Mg2＋-ATPase and total cell ATPase were significantly decreased 24 h after incubation（P0.05） and it was highly significant at 48 h post-inoculation（P0.01）.The activity of Ca2＋-Mg2＋-ATPase was sensitive to the PRV infection.