S0X2蛋白在喉癌组织中的表达及临床意义

Expression of SOX2 protein and its clinical significance in laryngeal carcinoma

目的:探讨SOX2在喉癌组织中的表达及与各临床病理因素的关系。方法:采用流式细胞术检测45例喉癌组织和45例相应癌旁组织中SOX2的表达,并以20例非喉癌患者喉部正常黏膜组织作为对照。结果:喉癌组织中SOX2蛋白的表达量及阳性表达率均明显高于癌旁组织及正常喉黏膜组织(P<0.05),癌旁组织与正常喉黏膜组织间的SOX2表达差异无统计学意义(P>0.05)。SOX2蛋白在喉癌组织中的表达与淋巴结转移、临床分期和分化程度差异有统计学意义(P<0.05);与临床分型、肿瘤大小、年龄和性别差异无统计学意义(P>0.05)。结论:SOX2蛋白的高表达可促进喉癌的发生和发展,其蛋白检测亦可作为判断喉癌浸润、转移以及预后的重要指标。

Objective：To investigate the expression of SOX2 in laryngeal carcinoma and analyze the relation of SOX2 and clinical factors. Method： We measured the expression of SOX2 protein in 45 laryngeal carcinoma fresh samples and 45 paracarcinoma tissues（cutting margin〉0.5 cm） with flow cytometer（Epics-XL Ⅱ ）, 20 normal la- ryngeal mucosa samples were also studied as controls. Result：The quantitative and qualitative expression of SOX2 protein in laryngeal carcinoma tissues was obviously higher than those in paracarcinoma and in normal laryngeal mucosa tissues respectively（P〈0.05）. There was no significant difference between the expression of paracarcino- ma and normal laryngeal mucosa tissues. In laryngeal carcinoma, the expression of SOX2 protein wasn＇t significant- ly related to patients＇ clinical classification, tumor size, smoking history, patientsrage and sex but related to metas- tasis,pathological grade and clinical stage. Conclusion：The high expression of SOX2 may contribute to the carcino- genesis and development of laryngeal carcinoma. It is an important index of judging metastasis and staging and prognosis of laryngeal carcinoma to measure the expression of SOX2 protein.