EGFL7在喉癌组织中的表达及临床意义

The study on clinical significance of the expression of EGFL7 in laryngeal squamous cell carcinoma

目的:探讨喉鳞状细胞癌(LSCC)组织中EGFL7的表达与喉癌侵袭转移的关系及其可能机制。方法:提取33例新鲜喉癌组织和33例相应的癌旁组织(PNTT)的总RNA,用RT-PCR方法测定EGFL7基因表达,Western-blot方法测定EGFL7蛋白的表达情况;用CD31抗体进行免疫组织化学检测喉癌组织中微血管密度(MVD),并分析EGFL7的表达及MVD与临床病理特征的关系。结果:33例新鲜喉癌标本中EGFL7mRNA和EGFL7蛋白表达均显著高于癌旁组织(P<0.01)。EGFL7mRNA和EGFL7蛋白表达具有显著相关性(r=0.786,P<0.01)。喉癌组织中EGFL7mRNA、EGFL7蛋白的表达水平和MVD与喉癌组织的临床分期、肿瘤的直径大小及有无淋巴结转移等密切相关(P<0.05),而与患者的性别、年龄、肿瘤原发部位及分化程度等无关(P>0.05)。结论:EGFL7可能通过影响血管生成参与了喉癌的发展。EGFL7蛋白可能成为预测喉癌的病情发展及指导临床治疗的标志物之一。

Objective：To investigate the association and mechanism of EGFL7 expression level with the inva sion and metastasis of laryngeal squamous cell carcinoma. Method： RT-PCR and Western blotting were used respec tively to detect the level of EGFI.7 mRNA and protein form 33 fresh laryngeal carcinoma tissues and matched para neoplastic non-tumor tissues. The immunohistochemistry technique was performed to determine microvessel densi- ty（MVD） in 33 tumor tissues.The association of EGFL7 expression and MVD with clinicopathological characteris- tics was analyzed. Result： EGFL7 mRNA and protein expression were both significantly higher in the tumor tissues than in the paraneoplastic non tumor tissues（P〈0.01）. Furthermore, the expression of EGFI.7 mRNA was high- ly correlated with the expression of EGFL7 protein（r=0. 786,P（0.01）. EGFI.7 expression and MVD were high- ly correlated with clinical stage, tumor size and the presence of lymph node metastasis（P〈0.05）, but was not correlated with the patients＇ gender, age, tumor sit and tumor site differentiation （ P〉 0.05 ）. Conclusion： EGFL7 may have a close correlation with the development of laryngeal carcinoma via its impact on tubulogenesis and vessel shape. EGFI.7 might serve as a tumor marker for assessing the progression of laryngeal carcinoma and a guide of clinical therapeutic decisions.