氢气生理盐水对创伤性胰腺炎大鼠氧化应激损伤作用的研究

Effects of hydrogen-rich saline on the oxidative stress injury of traumatic pancreatitis in rats

目的研究氢气生理盐水(HS)抑制氧化应激,减轻大鼠创伤性胰腺炎(TP)损伤的作用。方法将24只雄性Wistar大鼠随机分为假手术组(sham组)、TP组、生理盐水处理组(TP+NS组)和氢气生理盐水处理组(TP+HS组),利用压缩气流冲击暴露的大鼠胰腺,建立大鼠TP模型,于术后12h取胰腺组织观察其病理学变化,并观察胰腺内超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)变化。结果与TP+NS组比较,术后TP+HS组胰腺组织病理损伤情况明显改善(P<0.05),胰腺组织MDA水平较低(P<0.05),SOD活性和GSH含量较高(P<0.05)。结论 HS能减轻大鼠TP损伤,其机制可能与抑制胰腺创伤后氧化应激反应有关。

Objective To investigate the protective effects of hydrogen-rich saline by inhibiting the oxidative stress injury of traumatic pancreatitis in rats. Methods Twenty four male Wistar rats were randomly allocated into 4 groups： sham operation group, traumatic pancreatitis（TP） group,TP treated with normal saline（TP＋NS） group and TP treated with hydrogen-rich saline （TP＋ HS）group. An impact machine was used to produce compressed air to impact the exposed pancreas of rats, and animal models of traumatic pancreatitis were established. The changes in histopathology and the variation of SOD, MDA and GSH were observed 12 h after the operationResults The damage pancreas tissue in TP＋ HS group are slighter than that in TP＋ NS group after operation. The levels of plasma MDA are lower in TP＋ H S group than that in TP＋ N S group（P〈0.05）. The levels of plasma SOD and GSH are higher in TP＋HS group than that in TP＋NS group（P%0.05）. Conclusion The current results show that employing hydrogen-rich saline may reduce the damage of traumatic pancreatitis in rat. Its mechanism may be correlated with the inhibition of oxidant stress induced by traumatic pancreatitis.