TPH2基因多态性与单相抑郁及自杀行为关系

Association of single nucleotide polymorphism of tryptophan hydroxylase isoform gene with depression and suicidal behavior

目的探讨色氨酸羟化酶2(TPH2)基因G1463A单核苷酸多态性与单相抑郁症及自杀行为的关系。方法提取中国北方地区300例单相抑郁患者(病例组)和292名健康对照者(对照组)基因组DNA,采用TaqMan探针SNP基因分型技术测定包含TPH2基因G1463A位点的基因组DNA片段,检测病例组及对照组基因型的多态分布,应用SPSS 17.0软件进行统计分析。结果病例组与对照组SNPs基因型分布符合Hardy-Weinberg平衡定律(P>0.05);病例组携带的TPH2 G1463A基因型A/A、T/T、A/T频率分别为38.0%(114/300)、0.3%(1/300)和61.7%(185/300),对照组分别为50.0%(146/292)、40.1%(117/292)、9.9%(29/292),2组比较差异有统计学意义(χ2=231.626,P=0.000);病例组TPH2基因G1463A等位基因A、T频率分别为68.8%(413/600)、31.2%(187/600),对照组分别为55.0%(321/584)、45.0%(263/584),2组比较差异有统计学意义(χ2=24.155,P=0.000);病例组TPH2基因G1463A不同基因型的汉密尔顿抑郁量表(HAMD)总分及各因子得分比较差异均有统计学意义(P=0.000);病例组内有自杀行为的个体携带基因型A/A、T/T、A/T的频率及G1463A位点等位基因A、T频率比较差异均无统计学意义(P>0.05)。结论 TPH2基因G1463A单核苷酸多态性与单相抑郁症有明显关联,可能与抑郁症自杀行为易感性无关。

Objective To explore the association of single nucleotide polymorphism（SNP） analysis of a novel tryptophan hydroxylase isoform（TPH2） gene G1463A with depression and suicidal behavior. Methods We collected the specimens of peripheral blood from 300 depression patients and 292 controls in a population of northern China and extracted their genomic DNA. TPH2 gene G1463A was detected with TaqMan SNP genotyping assay and the association of polymorphisms of TPH2 gene with depression and suicidal behavior was analyzed using SPSS 17.0. Results The genotypic frequencies of the SNPs did not deviate from Hardy-Weinberg equilibrium in both case and control groups （P 〉0. 05 ）. Compared with the control group, significant differences of genotypes and alleles of TPH2 gene G1463A SNP were observed in patient group（38. 0% vs 50. 0% for A/A,0. 3% vs 40. 1% for T/T,61.7% vs 9. 9% for A/T, 68.8% vs 55.0% for allele A, and 31.2% vs 45.0% for allele T; P 〈 0.05 for all）. The genotypes of TPH2 gene G1463A in patient group were significantly associated with Hamilton Depression Rating Scale（HAMD） total score and factors （P = 0. 000）. There were no significant associations of genotypes and alleles of TPH2 gene G1463A with suicide behavior in patient group （ P 〉 0.05 ）. Conclusion TPH2 gene G1463A SNP significantly associates with unipolar depression ,but may not associate with the susceptibility of suicidal behavior in depression patients.