重组人乳铁蛋白小鼠喂养毒理学评价

Twenty-eight days feeding study on human lactoferrin expressed by cattle mammary bioreactor in mice

目的探讨牛乳腺生物反应器表达的人乳铁蛋白对小鼠亚急性毒性。方法断乳昆明小鼠48只,雌雄各半,随机分为2组,对照组给予AIN-93啮齿类实验动物纯合饲料,人乳铁蛋白组饲料用重组人乳铁蛋白替代AIN-93中的酪蛋白制成,进行小鼠28 d喂养试验,每周记录体重和进食量,实验结束时分析血液、取脏器称重并进行病理组织学检查。结果人乳铁蛋白组雄性小鼠终体重为(38.3±3.1)g,高于对照组的(34.9±4.3)g;人乳铁蛋白组雌性小鼠终体重为(31.2±2.4)g,对照组终体重为(31.2±1.9)g,2组差异无统计学意义;与对照组比较,人乳铁蛋白组小鼠食物利用率、血常规、血生化、脏器重量、脏体比和病理组织学检查结果未见明显改变。结论牛乳腺生物反应器表达的重组人乳铁蛋白对小鼠未见亚急性毒性作用。

Objective To investigate sub-acute toxicity of recombinant human lactoferrin（rhLf） from cattle mammary bioreactor in mice. Methods Groups of 12 male and 12 female weaning Kuming mice were fed with AIN-93 purified diets for laboratory rodents（ control group） or rhLf modified diets（ treated group） ,in which 20 g casein/100 g AIN-93 diet was substituted for 20 g rhLf/100 g diet,for a consecutive 28-day period. Body weight and food intake were recorded weekly. At the end of the study, blood was collected for routine and biochemical blood examination and organs were dissected and weighted; histopathological examination was accomplished simutaneously. Results The final mean body weight of rhLf-treated male mice was 38.3 g ±3.1 g,higher than that of the control （34. 9 g ±4. 3 g） significantly,but there was no significant difference between two female groups （ 31.2 g ± 2.4 g in the treated and 31.2 g ± 1.9 g in the control）. Food utilization rate, routine blood examination and biochemistry, absolute and relative weight of organs, and histopathology of organs of treated male and female mice were similar to those of the controls. Conclusion No evidence of sub toxicity was observed in the mice administered with recombinant human lactoferrin for 28 days.