沙门菌血清及脉冲场凝胶电泳分型

PFGE type and serotype of Salmonella strains

目的分析吉林省食品中分离的沙门菌血清型和脉冲场凝胶电泳(PFGE)型别,探讨吉林省食品中沙门菌污染的同源性。方法于2011年3月—2012年4月,对从市售生鸡肉和中式凉拌菜中分离出的28株沙门菌进行血清学和PFGE分子分型,采用BioNumerics version 6.0软件分析比较同源性。结果 28株沙门菌血清型分别为15株肠炎沙门菌、4株印弟安纳沙门菌、2株猪伤寒沙门菌、2株亚利桑那菌和5株血清未定型沙门菌;PFGE结果主要分为4个大群,其中1群包括14株,相似度为87.3%～100%;2群包括7株,分为2个亚群,2A亚群包括6株,相似度为91.9%～100%,2B亚群1株,相似度为73.6%;3群包括3株,相似度为88.9%～100%;4群包括4株,分为2个亚群,4A亚群包括3株,相似度为84.6%～94.7%,4B亚群1株,相似度为72.7%。结论吉林省食品中沙门菌血清型以肠炎沙门菌为主;PFGE结果显示相同血清型别菌株具有高度同源性。

Objective To analysze serotype and pulsed-field gel electrophoresis（PFGE） type of Salmonella strains isolated from food samples collected in Jilin province and to explore molecular polymorphism of Salmonella. Methods Totally 28 Salmonella strains were isolated from raw chicken meat and Chinese salad sampled in markets and genotyped with serologic method and PFGE. The homology of the strains was analyzed with BioNumerics 6.0. Results The serotypes of the 28 Salmonella strains were 15 Salmonella enteritidis ,4 Salmonella indiana ,2 Salmonella Cholerae-Suis ,2 Arizona, and 5 undetermined serotype. Based on PFGE results, the 28 strains were clasified into 4 major types, including 14 strains of type 1 with the similarity of more than 87.3% ,7 strains of type 2 （6 strains of 2A subtype with the similarity of more than 91.9%, one strain of 2B subtype with the similarity of more than 73.6% ）. Conclusion The main serotype of Salmonella isolated from food samples in Jilin province is Salmonella enteritidis. PFGE results show that the Salmonella strains of the same serotype are highly homologous.