粪便源指纹及其鉴别研究

Study on fingerprint and identification of origin of feces

利用气相色谱-质谱联用仪检测了人和32种动物粪便中以及5个不同排污口表层沉积物中10种粪固醇,以10种粪固醇含量和9个粪固醇比值为指标,分别应用层次聚类分析法对人和32种动物粪便以及5个沉积物的粪便污染来源进行了鉴别,结果表明:草食动物粪便中主要含C29-甾醇,5β-甾醇比5α-甾醇含量多,草食动物粪便的主要指示物是24-乙基粪醇和谷甾醇;肉食动物粪便中主要含C27-甾醇,胆固醇是肉食动物的主要指示物;杂食粪便中粪固醇组成多变;人粪便中主要含粪醇,占总粪固醇的51.5%;应用10种粪固醇含量和9个粪固醇比值以及层次聚类分析法,可以准确地鉴别人和32种动物粪便以及5个沉积物样品的粪便污染来源。

10 kinds of faecal sterols from feces in humans and 32 kinds of animals and surface sediments from 5 different ouffalls of sewages were analyzed by GC/MS. The origin of pollution from the feces was identified by the contents and the ratio values of faecal sterols with the hierarchical cluster analysis, respectively. The results showed that the profiles of C29-sterols were dominated, and 5β- stanols were greater abundance than 5α-stanols in the feces from herbivores, and 24-ethycoprostanol and sitosterol were as the principal faecal biomarker for herbivores. The profiles of C27 -sterols in the feces from carnivores were dominated and cholesterol was as the prin- cipal faecal biomarker of carnivores. It showed that the lots of sterols in faces from omnivores were varied, and the major of faecal sterol from feces of human was coprostanol constituted 51.5% of the total sterols in human faeces. It also showed that the origins of pollution from feces in humans and 32 kinds of animals and samples of surface sediments from 5 different outfalls of sewages could be accurately identified by 10 kinds of contents and 9 types of ratio values of faecal sterols with the hierarchical cluster analysis.