超高压电子显微镜三维图像重构的方法与初步结果

Three-Dimensional Reconstruction Using an Ultrahigh-Voltage Electron Microscope

建立了超高压电镜三维图像重构的方法与系统 ,首次在 2MV加速电压下进行了从获得二维电镜像到三维重构的尝试 .通过图像校正的方法 ,解决了单轴旋转试样夹中因试样高度变化引起的放大倍数和像转角变动的问题 ;提出了利用投影像的镜像对称关系来确定倾斜轴方向以及计算倾斜角度上相邻的两个投影像间的差来进行平移校直的方法 .大肠杆菌试样重构的实验结果表明 ,超高压电镜三维图像重构技术能够有效地再现厚试样的内部构造 。

This work is concerned with the reconstruction using an ultrahigh voltage electron microscope(Ultra HVEM) that makes electron tomography.Tomographic experiment at an accelerated voltage of 2 MV is reported for the first time. In image processing, correction is made for the magnification and image rotation alteration due to the specimen height change for single axis tilting.Presented is an alignment technique using the difference of two projection images. The three dimensional reconstruction of escherichia coli shows that Ultra HVEM tomography is effective for thick specimens. It is expected that application could be extended to biology and material science.