摘要
目的建立测定止咳宁嗽胶囊中苦杏仁苷含量的高效液相色谱法。方法用Zorbax Eclipse XDB-C18色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇-水(20∶80),检测波长为218 nm,流速为1.0 mL/min。结果苦杏仁苷的质量浓度线性范围为7.16～71.6μg/mL(r=0.999 5),平均回收率为99.21%,RSD为0.48%(n=6)。结论该方法专属性强、操作简便,可用于产品的质量控制。
Objective To establish a HPLC method for the determination of amygdalin in Zhikeningsou Capsule. Methods The Zorbax Eclipse XDB-C18 column（150 mm ×4. 6 mm,5 μm） was used with methanol-water （20：80） as the mobile phase. The UV detection wavelength was at 218 nm and the flow rate was 1. 0 mL/min. Results The linear range of amygdalin was 7. 16-71. 6 μg/mL （r = 0. 999 5）. The average recovery rate was 99.21% with RSD of 0. 48% （n = 6）. Conclusion The method is highly specific,simple to operate,and can be used to control the quality of this product.
出处
《中国药业》
CAS
2013年第2期19-20,共2页
China Pharmaceuticals
