摘要
试验用戊二醛法耦联AP和BSA,制得完全(包被)抗原AP-BSA,通过紫外扫描鉴定耦联成功,紫外吸光光度法测定得耦联比为5:1。通过方阵滴定试验确定,最佳包被浓度为4μg/mL,一抗的最适工作浓度为1:3000,二抗的最适工作浓度为1:400;通过二因素重复试验,确定用CBS做包被缓冲液,4℃下过夜为最佳包被条件。
AP was coupled with BSA by glutaraldehyde method to prepare complete （coated） antigen AP-BSA. It was identified that the coupling was successful by ultraviolet scanning and the results of determination by ultraviolet absorption spectrophotometry showed that the coupling rate was 5：1. Through phalanx titration test, it was confirmed that the best coating concentration was 4 ~g/mL, the optimum working concentration of the first antibody was 1：3 000 and the optimum working concentration of the second antibody was 1：400. It was confirmed by two-factor repeated test that the optional coating conditions were as follows ： using CBS as coating buffer and overnight below 4 ℃.
出处
《畜牧与饲料科学》
2013年第1期10-14,共5页
Animal Husbandry and Feed Science
基金
河南科技大学校青年基金研究项目(2006QN010)
