不同脱钙液对大鼠骨髓切片染色的影响

Effects of Different Decalcifying Solutions on the Staining of Bone Marrow Section of Rat

[目的]探讨不同脱钙液对Wistar大鼠胸骨骨髓切片染色效果的影响。[方法]取正常Wistar大鼠胸骨,采用10%中性福尔马林溶液充分固定,经流水冲洗后,分别浸入甲酸氯化铝脱钙液(A组)、5%甲酸脱钙液(B组)、5%硝酸脱钙液(C组)中进行充分脱钙后,再经流水冲洗,制作石蜡切片,HE染色,显微镜观察并评价其效果。[结果]①制片时间:5%甲酸组>甲酸氯化铝脱钙液>5%硝酸组;②HE染色效果:甲酸氯化铝脱钙液与5%甲酸组无显著差异,优于5%硝酸组。[结论]采用甲酸氯化铝脱钙液方法制作的大鼠胸骨骨髓切片,骨髓组织结构完整,细胞形态清晰,耗时较短,能够满足组织学评价的要求。

[Objective] The aim was to explore effects of different decalcifying solutions on the staining of sternum bone marrow section of Wistar rat. [Method] The sternum was collected from normal Wistar rat, and then fixed by 10% neutral formalin. After the samples were washed with flowing water, they were immersed in formic acid aluminum chloride decalcifying solution （A group）, 5% formic acid decalcifying solution （B group） and 5% nitric acid decalcifying solution （C group） to decalcify respectively, and then the samples were washed with flowing water again. After that, paraffin section was made and HE staining was carried out. Finally, the samples were observed under microscope. [Result] The effect on preparation section time showed as 5% formic acid group 〉 formic acid aluminum chloride group 〉 5% nitric acid group. The HE staining effect of formic acid aluminum chloride group had no significant difference with that of 5% formic acid group, but was better than that of 5% nitric acid group. [Conclusion] The bone sternum marrow section of rat made by formic acid aluminum chloride decalcifying solution had complete tissue structure of bone marrow, clear ceil morphology and short time, so it could satisfy the demand of histological evaluation.