冠心舒通胶囊对慢性心衰模型大鼠AT1、ERK2的影响

Effect of Guanxin-Shutong capsule on AT1, ERK2 expression in rats with chronic heartfailure

目的通过给药前后血管紧张素1型受体（ATl）及心肌细胞外信号调节激酶（E砌（2）信号通路在慢性心衰中的变化，探讨冠心舒通胶囊对慢性心衰模型大鼠ATI、ERK2的影响。方法以冠脉结扎法配合力竭式游泳、减食等方法造成大鼠慢性心衰动物模型。将造模成功大鼠按随机数字表法分为心衰模型组、赖诺普利组、益气中药组、活血中药组、益气活血中药组和冠心舒通胶囊组，没有进行左冠脉结扎术的假手术大鼠为正常组。利用实时荧光定量PCR技术、免疫组化法检测ATl、ERK2的变化。结果心衰模型组大鼠心肌ATl（2．528±0．27）、ERK2（2．658±0．328）表达升高，与假手术组比较，差异有统计学意义（P〈0．01）；与赖诺普利组比较，益气活血中药组、冠心舒通胶囊组大鼠心肌组织ATl、ERK2表达明显降低（P〉0．05），且中成药组疗效明显优于益气活血组（P〈0．01）。结论冠心舒通胶囊可通过抑制心肌组织中ATl、ERK2表达，抑制或逆转心室重构过程，达到治疗慢性心衰的目的。

Objective By investigating the effects of AT1 and ERK2 signaling pathway in CHF, to study the effect of Guanxin-Shutong capsule on AT1, ERK2 expression in rats with chronic heart failure （CHF）. Methods The CHF rat models were setup by coronary artery ligation, exhausting swimming and reducing feeding. Divided CHF rat methods into a model group, a lisinopril group, Qi-benefiting with Chinese medicine group, blood activating with Chinese medicine group, Qi benefiting and blood activating with Chinese medicine group （QBBA group） and Guanxin-Shutong capsule group. Rats without left coronary artery ligation were set for sham operated group. Real-time quantitative PCR technique and immunohistochemical method were adopted to detect AT1, ERK2 changes of CHF rats. Results AT1, ERK2 expression increased obviously in the model group compared with the sham operated group, and differences were statistically significant （P〈0.01）. After treatment, AT1, ERK2 expression reduced significantly in QBBA group and Guanxin-Shutong capsule group than the lisinopril group. （P〉0.05）. And therapeutic effect of Guanxin-Shutong capsule group was much better than other Chinese medicine treated group （P〈0.01）. Conclusion Guanxin-Shutong capsule would achieve the goal of treating chronic heart failure By inhibiting the expression of AT1 and ERK2 in organizations, and inhibiting or reversing ventricular remodeling process.