微流控芯片中金属离子印迹整体柱集成及用于Cd2＋富集和在线检测

Enrichment and Chemiluminescence Detection of Cd^(2+) in a Cd^(2+) Imprinted Polymer Monolith Integrated in a Polydimethylsiloxane Microchip

以Cd2+为模板,丙烯酰胺和4-乙烯吡啶为功能单体,二甲基丙烯酸乙二醇酯(EGDMA)为交联剂,环己醇/十二醇为致孔剂,采用原位聚合的方法在聚二甲基硅氧烷(PDMS)芯片中定位合成了Cd2+印迹整体柱。建立了微流控芯片中金属离子的富集-电泳-在线检测方法。通过扫描电镜和傅里叶红外光谱对合成的印迹整体柱进行了表征,证明合成的印迹整体柱具有良好的孔结构。将Cd2+印迹整体柱与固相萃取联用,优化了Cd2+印迹整体柱富集Cd2+的条件,并研究了Cd2+印迹整体柱的吸附性能和富集能力。在此基础上将Cd2+印迹整体柱与芯片电泳、鲁米诺-过氧化氢化学发光检测体系联用,实现了微流控芯片中Cd2+富集、分离以及检测的集成化。

We synthesized cadmium ion imprinted monolithic column in polydimethylsiloxane（PDMS） chip using in-situ polymerization technique, with the cadmium ion as a template, acrylamide and 4-vinylpyridine as functional monomer,ethyleneglycol dimethacrylate（EGDMA） as a crossiinking agent, cyclohexanol/dodecanol as porogen. A method for enrichment-electrophoresis-online detection of metal ion was therefore developed. The results from Scanning electron microscope（SEM） and fourier transform infrared spectroscopy （FTIR） indicated that the monolith has a good pore structure. We combined the cadmium ion imprinted monolithic column with solid phase extraction, and optimized the conditions for enrichment/elution of Cd2＋ and investigated the adsorption and enrichment capacity on ion imprinted monolith as well. Finally, the integration of the Cd2＋ enrichment,chip electrophoresis and chemiluminescence detection using luminol-hydrogen peroxide system has been realized on the microfluidic chip.