溪黄草及其不同提取物的红外光谱研究

Study on Rabdosia serra(Maxim.) Hara and Its Different Extracts by Fourier Transform Infrared Spectroscopy

采用一维红外光谱、二阶导数谱和二维相关红外光谱对溪黄草原药材及其不同溶剂提取物进行了红外光谱研究。结果表明:溪黄草原药材中含有酯类、芳香类和糖苷类等物质,溪黄草水提物中主要为糖类物质,乙醇提取物中主要为黄酮类物质,石油醚提取物中主要为酯类物质。红外光谱能够快速、简便地提供中药及其提取物中主要化学成分的宏观信息,从而为后续的化学成分分析和中药提取分离方法的改进和优化提供有效参考。

The objective of the present study is to analyze different extracts of Rabdosia serra （Maxim.） Hara by Fourier transform infrared spectroscopy （FTIR）, second derivative infrared spectroscopy and two-dimensional correlation spectroscopy（2D-IR） under thermal perturbation. The results show that the main chemical contents of Rabdosia serra （Maxim.） Hara medicinal materials are esters, aromatics and glycosides,etc. And the main chemical content of Rabdosia serra （Maxim.） Hara in its water extract, alcohol extract and ether extract are glycosides, flavonoids and esters, respectively. The spectroscopic technique described is a simple and rapid analytical technique, which could provide valuable information about chemical constituents of medicinal extracts for guiding further chromatographic analysis and separation improvement.