半整数生成函数法测定中性氨基酸的稳定常数

Determination of Stability Constant of Neutral Amino Acid with Bjerrum's Half-integer Formation Function Method

用电位滴定法作为实验手段,半整数生成函数法作为数据处理方法,分别采用氢氧化钠溶液和盐酸溶液直接滴定、氢氧化钠溶液返滴定、盐酸溶液返滴定等3种形式,对苏氨酸、甘氨酸、丙氨酸、丝氨酸、缬氨酸、苯丙氨酸、亮氨酸及异亮氨酸8种中性氨基酸的各级条件稳定常数进行了测定,所得结果与文献值吻合。

The method of Bjerrum＇ s half-integer formation function and potentiometric titration was applied for determination of stability constants of eight kinds of neutral amino acids,including threonine, glycine, alanine, serine, valine, phenylalanine, leucine and isoleucine. The applied ways of titration include direct titrations by sodium hydroxide and hydrochloric acid, back titration by sodium hydroxide, as well as back titration by hydrochloric acid. The results were conformity with those reported in literature.