Lipozyme RM IM催化棕榈油与甲醇醇解过程中酰基转移的研究

Acyl migration during methanolysis of palm oil and methanol catalyzed by Lipozyme RM IM

研究了无溶剂体系下1,3-位专一性酶Lipozyme RM IM催化棕榈油与甲醇醇解过程中酰基转移的情况,包括1,2-甘二酯酰基转移后形成1,3-甘二酯和2-单甘酯酰基转移后形成1(3)-单甘酯,探讨了反应底物摩尔比、反应温度和加酶量对酰基转移的影响。结果表明:醇油摩尔比小于3∶1时,酰基转移量随着反应时间的增加而增加;反应温度在50～60℃之间,酰基转移随着反应温度和反应时间的增加而增加;当加酶量从4%增加到8%时,酰基转移量随着反应时间的增加而增加。

Acyl migration during methanolysis of palm oil and methanol catalyzed by ! ,3 -positional specif- ic lipase（ Lipozyme RM IM） in solvent free system was studied, including acyl migration of 1,2 -diglycer- ide to 1,3 - diglyceride and 2 - monoglyceride to 1 （ 3 ） - monoglyceride. The eftcts of substrate molar ratio ,reaction temperature and enzyme dosage on the acyl migration were discussed. The results showed that when molar ratio of methanol to oil was lower than 3： 1, acyl migration increased with reaction time increasL ing; when the reaction temperature was in the range of 50 -60 ℃, acyl migration increased with the in- crease of reaction temperature and reaction time. When enzyme dosage increased from 4% to 8% , acyl mi- gration increased with reaction time increasing.