C6大鼠脑胶质瘤动物模型建立及病理观察

Animal Model Establishment and Pathological Observation of C6 Cerebral Glioma in Rats

目的建立简单易行、可靠稳定的大鼠C6脑胶质瘤模型,为研究脑胶质瘤的发病机制和防治方法提供操作平台。方法采用立体定向技术,将体外培养的大鼠C6胶质瘤细胞浓缩悬置,2.5×106个.25μL-1接种于SD大鼠的右侧尾壳核区,种植后连续观察大鼠的生存状态,并分别于7、14、21 d处死大鼠,立刻取脑,制作病理切片,HE染色,光镜下观察。结果大鼠接种C6胶质瘤细胞后,7 d左右生存状态良好,14 d左右出现较为明显的颅内高压症状,21 d左右时多数处于濒危状态。大体标本检查,18只大鼠中除3只意外死亡外,其余成瘤率100%,瘤体随着鼠龄的延长,中线结构明显移位,占位效应愈来愈明显。HE染色可明显观察到大鼠脑组织胶质瘤的形成。结论建立的C6大鼠脑胶质瘤动物模型可靠、稳定,其肿瘤生长特性及病理特征与人脑胶质瘤相似,可作为临床胶质瘤基础研究的理想模型。

Objective To establish the simple,feasible,reliable and stable C6 cerebral glioma model in rats so as to provide the platform for the study of the pathogenesis and the method of prevention and treatment of cerebral glioma.Methods The stereotaxic technique was adopted.C6 glioma cells in rats cultured in vitro were condensed and placed in suspension.C6 glioma cells（containing 2.5×10^6 C6 cells/25 μL）were implanted into the right caudate nucleus in SD rats.The survival conditions of the rats were consecutively observed.Separately,in the 7th,14th and 21st days of the implantation,the rats were sacrificed and the brains were collected to prepare the pathological sections.With HE staining,they were observed under light-microscope.Results In about 7 days,after C6 glioma cells implanted,the survival state of rats was good.In about 14 days,the intracranial hypertension was apparent.In about 21 days,most of the rats were in critical condition.In the gross specimen examination,except the accidental death of 3 rats,the tumor formation rate was 100% in the rest of 18 rats.Along with the aging of the rats,the tumor size was enlarged,the midline structure was migrated obviously and the occupied effect was getting marked.HE staining discovered the significant formation of cerebral glioma in the rats.Conclusion The animal model of C6 rat cerebral glioma is reliable and stable.The tumor growth property and pathological characteristics are similar to human cerebral glioma.Hence,the model is applicable satisfactorily for the clinical basic research of glioma.