脑卒中后抑郁大鼠海马、丘脑组织中BNDF、TrkB的表达变化及意义

Expression of BDNF and TrkB gene in hippocamopus and thalamus of post-stroke depression rats

目的观察脑卒中后抑郁(PSD)大鼠海马、丘脑组织中BDNF及其高亲和力受体原肌球蛋白受体激酶B(TrkB)的表达变化,并探讨其意义。方法将32只大鼠随机分为正常组、抑郁组、卒中组及PSD组各8只。正常组不干预;抑郁组采用孤养法制作抑郁模型;卒中组及PSD组采用线栓法致局灶性脑缺血(卒中);PSD组加慢性不可预见的中等应激刺激和孤养法制作PSD模型。采用RT-PCR法检测各组大脑海马及丘脑组织中的BNDF mRNA和TrkB mRNA。结果 PSD组海马BNDF mRNA、丘脑TrkB mRNA表达均明显低于对照组(P均<0.05);其余各组海马BNDF mRNA表达、丘脑TrkB mRNA表达比较均无统计学差异。结论 PSD大鼠海马、丘脑组织中BDNF及TrkB表达降低,二者可能参与了PSD的发病。

Objective To explore the expression of BDNF mRNA and high-affinity receptors TrkB mRNA in hippo- campus and thalamus of the post stroke depression rat model. Methods Focal cerebral ischemic rat models were made with thread embolization method. Post stroke depression rat models were established with comprehensive separately breeding and moderate unpredictable stress on this basis. RT-PCR was employed to detect gene expression of BDNF and TrkB in the hippocampus and thalamus. Results RT-PCR results showed that compared with normal control rats, the gene expression of BDNF and TrkB mRNA in the hippocampus decreased significantly in PSD group （ all P 〈 0.05）. Whereas there was no statistical difference compared with other groups （ all P 〉 0.05 ）. Conclusion The expression of BDNF probably play an important role in the development of PSD.