利用西瓜种子检测黄瓜绿斑驳花叶病毒

Utilizing Watermelon Seeds for Detection of Cucumber Green Mottle Mosaic Virus

以疑似感染黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)的西瓜植株上收取的种子为材料,提取总RNA为模板进行RT-PCR扩增,并对电泳后的目标条带进行回收测序,将测序结果提交genebank与已知序列比较,结果显示所扩增序列为CGMMV外壳蛋白(CP)基因中的一个片段,说明此方法可以成功检测出西瓜种子携带的CGMMV,可作为一种快速检测西瓜种子是否携带CGMMV的方法。

In the paper, we took the seeds harvesting from watermelons which were suspected of being infected with cucumber green mottle mosaic virus （CGMMV） as material, and extracted total RNA for RT-PCR amplification, then we recycled and sequenced the target bands, and submitted the sequencing results to genebank. The results showed that the amplified sequence was a fragment of the CGMMV coat protein （cp） gene, which indicated that the method used in the test could successfully detect whether the watermelon seeds carrying CGMMV or not, and the method was a rapid method of detecting CGMMV of watermelon seeds.