Box-Behnken设计优化黄芩超高压提取工艺

Optimization of Ultrahigh Pressure Extraction Technology of Baicalin from Scutellaria baicalensis by Box-Behnken Design

目的:应用Box-Behnken试验设计优选黄芩的超高压提取工艺。方法:以黄芩苷提取率为指标,采用单因素试验考察提取压力、提取次数、液固比等因素对黄芩超高压提取工艺的影响;Box-Behnken试验设计考察乙醇体积分数、液固比、提取压力对提取工艺的影响,优化工艺参数。结果:最佳提取工艺条件为乙醇体积分数72%,提取压力100 MPa,液固比100∶1,提取时间10 s,黄芩苷提取率102.65 mg.g-1。结论:优选的工艺提取率高、提取温度低、耗时短、能耗少,适用于黄芩的工业化提取。

Objective： To optimize ultrahigh pressure extraction （UPE） process of baicalin from Scutellaria baicalensis by Box-Behnken design. Method： With yield of baicalin as index, influence of extraction pressure, extraction times, liquid-solid ratio and other factors on UPE of S. baicalensis was investigated by single factor test; Box-Behnken design was employed to optimize extraction technology parameters with ethanol concentration, liquid-solid ratio and extraction pressure as independent variables. Result： Optimum extraction technology conditions were as following： extracted 10 s with 100 times the amount of 72% ethanol by extraction pressure of 100 MPa, yield of baicalin was 102.65 mg· g-1 Conclusion： Optimized technology had higher yield, lower extracting temperature, shorter extacting time and less power consumption, it was suitable for industrial production of S. baicalensis.