摘要
目的:探讨天麻水溶性成分的煎出量与煎煮时间的关系。方法:采用高效液相色谱法,phenomenex C18(4.6 mm×250 mm,5μm)色谱柱,流动相乙腈与0.2%磷酸,梯度洗脱,流速1 mL.min-1,检测波长220 nm。结果:天麻素在0.354 4～3.544μg线性关系良好(r=0.999),平均回收率为99.4%,RSD 1.2%,天麻在煎煮3 h时天麻素等6个主要成分的煎出量最大。结论:天麻药材的最佳煎煮时间为3 h,与传统要求的久煎相吻合。
Objective： To investigate the relationship between the amount of water-soluble components of gastrodia and the boiling time. Method： A phenomenex Cts （4.6 mm x 250 mm, 5 Ixm） column was adopted. The mobile phase consisted of acetonitrile （A） and 0.2% phosphoric acid solution （B） in gradient elution mode. The flow rate was 1.0 mL ~ rain-＇ The detection wavelength was set at 220 nm. Result： The linear range of gastrodin was 0. 354 4-3. 544 p,g （r 〉 0. 999）. The average recovery was 99.4% , RSD 1.2%. The maximum amounts of six major components including gastrodin were decocted 3 hours. Conclusion： The best time of decoction of Gastrodiae Rhizoma was 3 hours, consistent with traditional requirements.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第3期83-85,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81102530)
