HPLC同时测定络石藤中5个化学成分的含量

Simultaneous Determination of Five Compounds in Trachelospermum jasminodes by HPLC

目的:建立测定中药络石藤中络石苷元-4'-O-β-龙胆二糖苷、络石苷、野漆树苷、络石苷元及木犀草素含量的HPLC方法。方法:75%甲醇提取络石藤药材,微孔滤膜(0.45μm)过滤,HPLC测定,采用Alltima C18色谱柱(4.6 mm×250mm,5μm),以0.5%HAc-甲醇为流动相梯度洗脱,流速1.0 mL.min-1,检测波长270 nm,采用外标法峰面积计算含量。结果:5种成分均达到基线分离,线性关系良好,精密度RSD<2.0%。该法成功的用于络石藤药材中5种成分含量的测定。药材中络石苷元-4'-O-β-龙胆二糖苷、络石苷、野漆树苷、络石苷元及木犀草素的平均回收率分别为98.27%,97.30%,97.20%,96.89%,96.48%。结论:方法操作简单、结果准确,具有较好的重复性和稳定性,为络石藤药材质量控制提供了依据。

[Abstract] Objective： To establish a method for determination of trachelogenin--4＇-O-β--gentiobioside, tracheloside, rhoifolin, trachelogenin and luteolin in Trachelospermum jasminoides. Method： The Alltima C,s column （4.6 mm x250 ram, 5 ixm） was used; the mobile phase consisting of 0.5% HAc-water. The flow rate was 1.0 mL .min-1; the detection wavelength set at 270 nm and content calculated with external reference method. Result： The five compounds were well separated. Calibration curves showed good linear relationship, RSD 〈 2.0%. The average recoveries of trachelogenin--4＇-O-β-gentiobioside, tracheloside, rhoifolin, trachelogenin and luteolin were 98.27% , 97.30% , 97.20% , 96. 89% and 96.48% respectively. Conclusion： The developed method is simple, accurate, and repeatable, which can be readily used as valid tool for the quality control of T. Jasminoides.