降糖消脂片对KK-A^y糖尿病肥胖小鼠胰岛素受体及底物表达的影响

Effect of Jiangtang Xiaozhi Tablets on Expression of Insulin Receptor β and Insulin Receptor Substrate-1 in KK-A^y Transgenic Mice Model of Diabetes Mellitus

目的:建立糖尿病肥胖小鼠模型,观察降糖消脂片对模型小鼠胰腺胰岛素受体及底物表达的影响。方法:采用KK-Ay转基因小鼠,高脂饲料喂养造成糖尿病肥胖小鼠模型。对照组为同龄C57小鼠,成模的KK-Ay小鼠分为模型组、吡格咧酮组、降糖消脂片高、中、低剂量组(10,5,2.5 g.kg-1)(11只/组),连续灌胃给药(ig)8周。每周称体重及摄食量,隔周测血糖。药后8周取胰腺作病理切片,并取胰腺组织检测胰岛素受体及底物表达。结果:降糖消脂片各剂量组明显减轻糖尿病肥胖小鼠体重,降低血糖。病理结果显示:降糖消脂片可减少变性及凋亡的胰岛细胞。糖尿病肥胖模型组小鼠胰腺组织胰岛素受体β(insulin receptorβ,InsRβ)和胰岛素受体底物-1(insulin receptor substrate 1,IRS-1)的表达低于对照组。降糖消脂片可有效诱导InsRβ和IRS-1表达。结论:降糖消脂片对KK-Ay糖尿病肥胖小鼠有明显的治疗作用,并可诱导胰岛素受体及底物表达。

Objective： To observe effects of Jiangtang Xiaozhi （JTXZ） tablets on expression of insulin receptor β （InsRβ） and insulin receptor substrate-1 （IRS-1） in KK-Ay transgenic mice model of diabetes mellitus （DM）. Method： KK-Ay transgenic mice were used and fed with high-fat diet to induce hyperglycemia as a model of DM obesity. The C57mice with same age of KK-Ay transgenic mice were used as a control group. Thereafter, Fifty-five mice with DM obesity were divided into 5 groups （n = 11 ） including model group without any treatment, pioglitazone hydrochloride tablets treatment group as a positive control group, and JTXZ tablets groups at high, middle and low treatment doses, and were continuously given JTXZ tablets by intragastric administration for 8 weeks. All mice were sacrificed after treatment of 8 weeks. The pathology examination of abdominal salivary gland was performed. The expression of InRβ and IRS-1 were deteced, respectively. Result： In the JTXZ tablets treatment group, compared with that in KK-Ay transgenic mice model with DM obesity, the pancreatic pathological changes were ameliorated.significantly. Expression of InRβ and IRS-1 were increased in each treatment group by JTXZ tablets, respectively. Conclusion： JTXZ tablets have obvious effects of increasing levels of InRβ and IRS-1 in KK-Ay transgenic mice model with DM obesity.