莪术对妊娠期大鼠子代发育毒性研究

Developmental Toxicity of Offspring Induced by Exposure Pregnant Rats to Curcumae Rhizoma

目的:观察妊娠期莪术暴露对大鼠子代发育的影响。方法:Wistar雌性大鼠随机平均分为血瘀证组和正常组,采用皮下注射盐酸肾上腺素及冰水浴复制大鼠血瘀证模型。造模结束后,雌雄2∶1合笼,受孕后正常组和血瘀证组孕鼠随机分为1.4,2.8,5.6 g.kg-1莪术组和对照组。于妊娠6～19 d连续ig给予不同剂量的莪术水煎液。检测仔鼠出牙、开眼、张耳和出毛等生理发育指标,以及平面翻正、断崖回避、负趋地性、前肢悬挂、空中翻正和听觉惊愕等神经行为指标。结果:正常大鼠和血瘀证大鼠给予莪术暴露后两者的负趋地性和断崖回避指标存在差异。在5.6 g.kg-1莪术组,正常大鼠的负趋地性和断崖回避达标日龄负趋地性为(7.00±0.76)d;断崖回避为(5.85±0.81)d,较空白对照组负趋地性为(6.65±0.76)d;断崖回避为(5.45±0.96)d明显延迟(均P<0.05),而血瘀证模型大鼠的仔鼠各项指标与对照组比较无显著性差异。结论:莪术对正常大鼠和血瘀证模型大鼠的神经行为发育的影响具有差异,对正常大鼠的毒性效应更明显。

Objective： In order to evaluate the effect of developmental toxicity of offspring pregnant rats treated with Curcumae Rhizoma. Method： Wistar female rats were randomized into normal group and blood-stasis model group. A model of blood stasis model in rats by injecting adrenalin and being immersed in ice water was used. The rats were mated and pregnant rats of two groups were randomized into the low, medium and large dose sub-groups and control group. Curcumae Rhizoma were orally administered to pregnant rats from gestational day （GD） 6 through 19 at dose levels of 0, 1.4, 2.8, 5.6 g kg-1. Physical development and neurobehavioral function were examined. Result： There was a difference of neurobehavioral function toxicity induced by Curcumae Rhizoma between normal and blood-stasis pregnant rats. Compared with the blank group, the pass time of cliff avoidance test and negative geotaxis reflex of normal rats delayed significantly. We could not find any significant changes in that of blood-stasis rats. Conclusion： Neurobehavioral toxicity of offspring of the blood-stasis pregnant rats to Cureumae Rhizoma is weaker than that of the normal pregnant rats, confirmed the rationality of evaluating the toxicity of Chinese medicines based on TCM syndrome theory.