血管瘤组织总ERK1/2及磷酸化ERK1/2的免疫印迹检测

Immunoblotting Detection of Total ERK1/2 and Phospho-ERK1/2 in Hemangioma

目的检测细胞外信号调节激酶1/2(总ERK1/2)及磷酸化ERK1/2(p-ERK1/2)在血管瘤组织中的表达并探讨其意义。方法应用免疫印迹法检测10例增生期血管瘤组织和8例退化期血管瘤组织总ERK1/2及p-ERK1/2的蛋白表达。结果增生期血管瘤组织总ERK1/2的蛋白表达(0.87±0.14)与退化期血管瘤组织总ERK1/2的蛋白表达(0.79±0.05)差异无统计学意义(P>0.05);增生期血管瘤组织p-ERK1/2的蛋白表达(0.44±0.04)明显高于与退化期血管瘤组织p-ERK1/2的蛋白表达(0.23±0.08),差异有统计学意义(P<0.01)。结论血管瘤增生与ERK活性增高及ERK通路激活有关。

Objective To investigate protein expression of total extraeellular sigual-regulated kinasel/2 （ total ERK1/ 2） and phospho-extraeellular signal-regalated kinasel/2 （p-ERK1/2） in hemangiomas, and to explore their role in the development of hemangiomas. Methods Total ERK1/2 and p-ERK1/2 protein levels in 10 pro- liferating phase hemangiomas and in 8 involuting phase hemangiomas were assessed by Immunoblotting. Re- suits There was no significant difference between total ERK1/2 protein levels（0.87±0.14）in proliferating hemangioma and those（0.79 ± 0.05 ） in involuting hemangioma （ P 〉 0.05 ）. The p-ERK1/2 protein levels （ 0.44 ± 0.04 ） in proliferating hemangioma were significantly higher than those （ 0.23 ± 0.08 ） in involuting hemangioma （P 〈 0.01 ）. Conclusion The aetivation of ERK1/2 and ERK signalling pathway may be re- lated with hemangiomatous proliferation.