氨甲环酸外用对小鼠角质形成细胞分化脱落相关蛋白表达水平的影响

Effect of Topical Tranexamic Acid on Expression of Proteins Involved in Differentiation and Desquamation of keratinocyte

目的研究外用氨甲环酸对皮肤屏障功能的影响及其分子机制。方法在BALB/c小鼠背部制备慢性皮炎模型,皮损处分别涂抹5%氨甲环酸水溶液(氨甲环酸组)及蒸馏水(基质组),1次/d,共14d。测定两组小鼠经表皮水分丢失量及丝聚蛋白、外皮蛋白、转谷氨酰胺酶1和激肽释放酶7的蛋白及mRNA表达情况。结果与基质组[(12.10±1.33)g/(m2·h)]相比,氨甲环酸组小鼠背部皮损处经表皮水分丢失量[(9.78±0.54)g/(m2·h)]显著降低(P=0.003),而表皮丝聚蛋白、外皮蛋白表达强度增强。氨甲环酸组丝聚蛋白(269.76±140.02vs1335.80±396.34)、激肽释放酶7(113.49±71.80vs659.44±368.33)的mRNA表达量均显著低于基质组(P<0.05)。结论氨甲环酸可能通过影响某些角质形成细胞分化脱落相关蛋白的表达水平以促进皮肤屏障功能的修复。

Objective To investigate the mechanism of topical tranexamic acid and its effects on skin barrier. Methods In the study, a chronic dermatitis was induced in BALB/c mouse, followed by the application of 5% tranexamic acid （tranexamic acid group） or distilled water （vehicle group） to the eruption once a day for fourteen days. Then data were obtained from two groups of mice with respect to their Transepidermal Water Loss （TEWL） , the expression of protein and mRNA of Filaggrin （FLG）, Involucrin （IVL） , Transglutami- nase 1 （TGM1） and Kallikreiu7 （KLK7）. Results Compared with the vehicle group [ （ 12. 10 ± 1.33 ） g/（ m2· h） ], mice in the tranexamic acid group had significantly lower TEWL[ （9.78±0.54）g/（ m2 · h） ] （ P = 0. 003 ） , and increased expression of FLG and IVL in epidermis. The mRNA level of FLG （ 269.76 ± 140.02 vs 1 335.80 ± 396.34） and KLK7 （ 113.49 ± 71.80 vs 659.44 ± 368.33 ） were significantly lower in the tranexamic acid group than that in vehicle group （ P 〈 0.05 ）. Conclusion Tranexamic acid may help the recovery of skin barrier by changing the expression of some protein involved in keratinocyte differentiation and desquamation.