TLR9在初发和复发尖锐湿疣皮损及外周血T细胞亚群中的表达

TLR9 on CA Skin Lesions or in Peripheral Blood T Lymphocyte Subsets of Primary and Recurrent CA

目的检测初发和复发尖锐湿疣(CA)患者皮损及外周血T细胞亚群中TLR9的表达情况,并探讨TLR9在HPV感染和CA复发机制中的作用。方法分别选用30例初发CA和27例复发CA患者皮损,并以28例正常人皮肤石蜡切片作为正常对照组。采用双色免疫荧光抗体染色流式细胞术检测入选者外周血T细胞中TLR9的表达。结果初发CA组和复发CA组皮损颗粒层和棘层TLR9表达(1.38±0.72和2.06±0.84)和外周血CD3+CD4+T细胞内TLR9表达[(11.4±3.2)%和(14.6±2.4)%]显著高于正常对照组[0.97±0.43和(6.2±2.1)%];另外,复发CA患者外周血CD3+CD4+T细胞内TLR9的表达也显著高于初发CA组;以上差异均有统计学意义(P均<0.05)。但是该两组患者的外周血CD3+CD8+T细胞内TLR9的表达与正常对照组差异不显著(P>0.05)。结论 CA皮损及外周血CD3+CD4+T细胞内TLR9表达上调可能是HPV病毒感染的识别受体,并参与机体的抗HPV免疫应答。TLR9可能在CA复发机制中发挥着重要的作用。

Objective To explore the role of TLR9 in HPV infection and condyloma acuminatum （CA） recurrence mechanism through detecting the expression of TLR9 on skin lesions or in peripheral blood T lymphocyte subsets of primary and recurrent CA patients. Methods 30 cases primary CA patients and 27 cases recur- rence CA patients with skin lesions were collected, 28 cases of paraffin sections of normal skin were used as normal control. Two-color immunofluorescence antibody staining by flow cytometry technique was used to de- tect TLR9 expression in normal, primary and recurrent CA patients, peripheral blood T ceils. Results In the primary and recurrent CA group, the expression TLR9 on the granular layer and acantholysis of skin le- sions （1.38 ± 0.72 and 2.06 ±0.84） and in CD3 ＋CD4＋T ceils [（11.4 ±3.2）% and （14.6 ±2.4） % ] were significantly higher than that in normal control group [ 0.97 ± 0.43 and （6.2 ± 2.1 ） % ]. In addition, the expression of TLR9 in peripheral blood CD3 ＋ CD4 ＋ T cells of recurrence CA patients was significantly increased compared with the primary CA patients. The above differences were statistically sig- nificant （ all P 〈 0.05 ）. However, the differences of the TLR9 expression between in peripheral blood of the patients CD3 ＋ CD8 ＋T cells and that in normal control group were not significant （P 〉 0.05 ）. Conclu- sion The upregulation of TLR9 expression in CA skin lesions and peripheral blood CD3 ＋ CD4 ＋ T cells may serve as a recognition receptor for HPV infection, and involved in the body＇ s anti-HPV immune response. TLR9 may play an important role in the CA recurrence mechanism.