PR(+)/PR(-)乳腺癌差异表达microRNAs潜在作用通路的生物信息学分析

Bioinformatics analysis of microRNAs regulated potential signaling pathways in breast cancers with different PR status

目的通过对乳腺癌中与孕激素受体(PR)状态相关的microRNAs差异表达谱进行生物信息学分析,探讨microRNAs在PR表达状态中可能参与的调控机制,为深入研究乳腺癌不同分子亚型的分化提供新思路。方法利用文献挖掘方法找到与PR状态相关的microRNAs差异表达数据集,然后利用生物信息学方法(Targetscan软件和DAVID数据库)预测microRNAs的靶基因,再对靶基因进行基因本体论(GO)富集和通路分析。结果通过文献挖掘找到3个差异表达microRNAs数据集,生物信息学分析获得3个相应靶基因集和1个靶基因合集。靶基因的GO分类主要涉及细胞内的信号级联、蛋白氨基酸磷酸化、蛋白质甲基转移酶活性及转录因子活性等生物学过程及分子功能。通路分析发现3条KEGG信号通路和3条BIOCARTA代谢通路可能参与不同PR表达状态的调节。结论通过生物信息学方法,初步分析了microRNAs在不同PR表达状态中可能参与调控的信号和代谢通路,为进一步探索microRNAs在乳腺癌不同分子亚型分化的调控机制奠定基础。

Objective To perform bioinformatic analysis on the aberrant expression of mieroRNAs in PR （＋）/PR （-） breast cancers,so as to explore the possible regulatory signaling pathways of different progesterone receptor status. Methods PR genes related microRNAs were retrieved through TargetScan and literature mining. Then, an analysis on gene sets was carried out by GO overrepresentation and pathway analysis using DAVID database. Results Three sets of aberrantly expressed microRNAs were obtained. Results from the gene ontology category indicated that these target candidates mainly participate in intracellular signaling cascade, protein amino acid phosphorylation, protein methyltransferase activity, and transcription activity.Pathway analysis showed that these target genes were mainly involved in 3 KEGG signaling pathways and 3 BIOCARTA pathways. Conclusions This study analyzed the influence of microRNAs in the cellular functions and pathways in breast cancer cells of different PR status. The result inay be used for the biological interpretation of microRNAs profiling data in patients with different sub-type of breast cancers.