摘要
目的:研究地塞米松预处理对液氮深低温冻存猪角膜内皮细胞的影响。方法:选择新鲜猪角膜,以13mm直径环钻制取角膜标本,依次放入4种冷冻保护液中冷平衡,"简化四步法"程序降温后放入液氮中保存,使用前40℃水浴复温。地塞米松作用组以同法制备植片,前三步冷平衡同前,最后分别放入含0.01,0.03,0.05mg/mL地塞米松保护液中4℃孵育18h,再程序降温液氮冻存。复温后用于锥兰-茜素红染色,观察内皮细胞存活情况。结果:内皮细胞染色结果示:程序冻存组和地塞米松作用组的内皮细胞存活率均超过90%,差异比较无统计学意义(P>0.05)。结论:在0.01~0.05mg/mL浓度范围内,未见地塞米松毒性作用,内皮细胞维持正常六角形形态,内皮细胞存活率超过90%。
AIM: To study the influences of dexamethasone to cryopreserved cornea endothelial cells. METHODS: Fresh pig corneas were drilled down by 13mm-diameter trephine. Then the grafts were put into four kinds of cool cryoprotective media by turns and equilibriumed with ' simplified four - step' method. All grafts were thawed in 40℃. All dexamethasone pretreatment corneas were additionally incubated in the last cool cryoprotective medium which had 0.01mg/mL, 0.03mg/mL or 0.05mg/mL dexamethasone for 18 hours in 4℃ before cryopreservation. Corneas thawed were stained by typan blue and alizarin Bordeaux. Survival rates of endothelia cells were observed. RESULTS: There were no significant differences in endothelia survival rate among rate-freezing group and every dexamethasone pretreetment group. CONCLUSION: In 0.01 -0.05mg/mL of concentrations, dexamethasone shows no toxic effects. Survival rate of endothelial cells was more than 90%.
出处
《国际眼科杂志》
CAS
2013年第2期260-261,共2页
International Eye Science
关键词
角膜移植
内皮细胞
深低温冻存
地塞米松
corneal transplantation
endothelial cell
immunogenicity cryopreservation
dexamethasone