摘要
对HPLC法和ELISA法检测牛奶样品中黄曲霉毒素M1(AFM1)残留量进行了比较,探讨了牛奶中黄曲霉毒素M1的最佳检测方法。结果显示,对于已知加标样品(阳性),HPLC法回收率为68%～85%;ELISA法回收率为97%～109%,HPLC法和ELISA法具有良好的相关性。ELISA法检验成本较低、检测迅速,但可能出现少量的假阳性和假阴性,故可用于待测样品的初筛;HPLC法检测结果准确,但检测费用较高,适用于黄曲霉毒素M1残留的确证。
The method for detecting aflatoxin M1 in milk was studied by comparison of HPLC and ELISA method. The recovery rates of HPLC were 68%-85% and the recovery rates of ELISA were 97%-109%. Detection results of HPLC and ELISA had good correlation. ELISA method had low cost, rapid detection, it could be used for preliminary screening of aflatoxin M1. HPLC method was accurate but it had high cost of testing, it is suitable for conclusive evidence of aflatoxin M1 residue.
出处
《化学分析计量》
CAS
2013年第1期48-50,共3页
Chemical Analysis And Meterage
