高效液相色谱法快速测定紫贻贝中的牛磺酸

Fast determination of taurine in Mytilus edulis Linnaeus by high-performance liquid chromatography

目的:建立用高效液相色谱(HPLC)快速测定紫贻贝中牛磺酸含量的方法。方法:应用邻苯二甲醛(OPA)为衍生试剂进行柱前衍生,采用甲醇、乙腈和水(10:10:80,v/v/v)为流动相,色谱柱为Agilent Zorbax300SB-C18(4.6mm×250mm,5μm),荧光检测:Ex为330nm,Em为530nm,柱温30℃,流速0.8mL/min,进样20μL分析检测牛磺酸含量。结果:牛磺酸在4.0～20.0μg/mL浓度范围内有良好线性关系,相关系数R2为0.9998,回收率范围在96.65%～101.20%,RSD=1.57%。结论:该方法的样品前处理与衍生化简便、易操作,牛磺酸出峰时间为6.752min,其含量为3673.631mg/kg,有良好的精确度和准确度,分析时间短,可快速检测紫贻贝中的牛磺酸。

Objective：To establish the method for fast determination of taurine in Mytilus edulis Linnaeus by high performance liquid chromatography（HPLC）. Methods：The content of taurine in Mytilus edulis Linnaeus was determined by HPLC at the FLD detector excitation and emission wavelengths of 330nm and 530nm through Agilent Zorbax 300SB-C18（4.6mm×250mm,5μm） column with pre-column o-pthaldialdehyde derivatization. The column temperature was 30℃,methanol-acetonitrile-water（10：10：80） was used as the mobile phase at the flow rate of 0.8mL/min. The injection volume was 20μL. Results：The taurine was in the range of 4.0N20.0μg/mL with good linear correlation,correlation coefficient R2=0.9998,the recoveries were 96.65% ~101.20% ,RSD = 1.57%. Conclusions：The determination method was accurate with good reproducibility. The retention time of taurine was 6.752min,the taurine content was 3673.631mg/kg wet weight. The method can be used for taurine contents determination in Mytilus edulis Linnaeus.