斑点叉尾鮰鱼皮胶原蛋白的理化特征研究

Physical and chemical properties of collagen from the skin of channel catfish

选用斑点叉尾鮰(Ictalurus punctatus)鱼皮为材料,用乙酸和乙酸-胃蛋白酶,分别提取鱼皮中的酸溶性胶原蛋白(acid-solubilise collagen,ASC)和酶溶性胶原蛋白(pepsin-solubilise collagen,PSC),并对其理化性质特征进行研究。研究发现,提取得到的ASC纯度高达93.11%,PSC纯度高达93.46%;紫外吸收分析表明,ASC和PSC的吸收峰值均在233nm处;蛋白图谱中两种胶原蛋白均由两种不同的α链(α1)2α2组成,具备I型胶原蛋白的特征;ASC和PSC的傅里叶红外图谱相似,具有完整的三螺旋结构;ASC的变性温度为34.2℃,PSC的变性温度为33.9℃。

The acid-soluble collagen（ASC） and pepsin-soluble collagen （PSC） were extracted from skin of channel catfish by the method of acid and acid-pepsin ,and their physical and chemical characterization were analysed. The study indicated that the ASC had high purity of 93.11% and PSC of 93.46% ,the analysis of UV spectra of ASC and PSC showed that the maximum absorption was obtained at 233nm. Both ASC and PSC consisted of two different α-chains （α1）2α2 were characterized as type I. Fourier transform infrared （FTIR） spectra of both ASC and PSC were almost similar with whole triple-helical structure. Transition temperature of ASC and PSC was 34.2℃ and 33.9℃.