紫苏乳状液酶解破乳工艺的研究

Research of hydrolysis de-emulsification of emulsion from enzyme-assisted aqueous extraction processing of perilla frutescens

利用水酶法进行紫苏油脂的提取时会形成乳状液,乳状液是由油脂、蛋白、磷脂以及碳水化合物共同组成的稳定的乳化体系,由于乳状液中富含油脂,因此如何对乳状液进行有效的破乳是提高水酶法油脂得率的关键。以水酶法提取紫苏油过程中所形成的乳状液为研究对象,通过二次酶解的方法对其破乳工艺进行研究,考察了酶解时间、酶解温度、pH以及加酶量对紫苏油脂回收率的影响,并采用响应面法对破乳工艺进行优化。结果表明,在Protex6L酶解时间1.6h,酶解温度62.6℃,pH9.4,加酶量1.9%条件下,紫苏油脂回收率为85.52%;利用酶解进行破乳,此方法可行,操作安全,具备工业化应用潜力。

Emulsion was formed during the process of enzyme-assisted aqueous extraction of perilla frutescens seeds oil. It is stabilized by oil,proteins,phospholipids and carbohydrates,which compose a state emulsified system, as it is rich in oil ,therefore, how to demulsify effectively the emulsion ,which is the key to increase the oil yield of enzyme-assisted aqueous extraction processing（EAEP） of oil. Emulsion formed in the emulsion from enzyme-assisted aqueous extraction processing（EAEP） of Perilla Frutescens Seeds oil was taken as study object,and econdary hydrolysis de-emulsification was adopted to demulsify the emulsion,the effect of hydrolysis time,hydrolysis temperature,hydrolysis pH and enzyme dosage on oil recovery yield. Response surface methodology （RSM） was applied to optimize the demulsification technology. Optimal hydrolysis conditions were Protex 6L,hydrolysis time of 1.6h,hydrolysis temperature of 62.6℃,hydrolysis pH of 9.4 and enzyme dosage of 1.9%. Under these optimal hydrolysis conditions,the maximum oil recovery yield was up to 85.52%. For hydrolysis de-emulsification,it was a good method that feasible,safe operation,and potential for industrial application.