植物甾醇对肉鸡肠道氧化还原状态的影响

Effects of phytosterol on intestinal redox status of broilers

目的:研究植物甾醇(phytosterols)对肉鸡肠道氧化还原状态的影响。方法:选用105只1日龄雄性罗斯肉鸡(44g)随机分为对照组(基础日粮)和实验组(在基础日粮中分别添加0.015‰、0.020‰、0.025‰的低羟基值植物甾醇和0.025‰、0.050‰、0.075‰的高羟基值植物甾醇),共7个处理组,每组3个重复,每个重复5只鸡。结果:与对照组相比,0.015‰、0.050‰和0.075‰植物甾醇添加组使肉鸡回肠总抗氧化能力(T-AOC)提高。各植物甾醇添加组使反映机体氧化还原状态的还原型与氧化型的谷胱甘肽的比值(GSH/GSSG)显著升高(p<0.05),使肉鸡十二指肠、空肠脂质过氧化产物丙二醛(MDA)的水平降低但不显著。结论:植物甾醇能通过清除自由基、恢复氧化还原平衡、解除慢性氧化应激,从而保护机体免受氧化损伤。

Objective：To research the effect of phytosterols on intestinal redox state of broiler chickens. Methods. One hundred and five male broiler chickens（44g each） were randomly divided into 7 groups,and each group was assigned to 1 of 3 treatments. Birds in each group were kept in three coops（5 broilers per coop）. All broilers were offered the same basal diet with added phytosterols at levels of 0（control）,0.015‰,0.020‰, 0.025‰ phytosterols of low hydroxyl valve and 0.050‰,0.025‰,0.075‰ phytosterols of high hydroxyl valve. Results：Indicated that compared with control treatment,broilers fed diets supplemented with phytosterols at levels of 0.015‰,0.050‰,0.075‰ could increase the level of total antioxidant status（T-AOC） of ileum. Broilers fed diets supplemented with phytosterols could significantly increase the level of GSH/GSSH,which reflects the redox status of broilers （p〈0.05）. Meanwhile,it could also reduce the level of malondialdehyde（MDA） of duodenum and jejunum,but not significantly. Conclusion：Adding phytosterols could clean out free radicals, recovery the redox balance and relieve oxidative stress,which protected the body from oxidative damage.