NAC壳聚糖膜干预大鼠腹部术后腹膜核转录因子Sp1和NF-κB的表达

Downregulation of nuclear transcription factors Sp1 and NF-κB expressions by N-acetyl cysteine chitosan during the postoperative peritoneal adhesion formation in rats

目的探讨NAC(N-乙酰半胱氨酸)生物膜对大鼠腹膜损伤粘连修复演变过程中核转录因子Sp1和NF-κB活化的影响。方法建立大鼠腹部回盲部手术动物模型,术中腹膜创面覆盖留置NAC壳聚糖多孔膜,术后腹腔镜连续观测、提取相应腹膜粘连组织,监测腹膜粘连分级变化。大鼠分4组:正常腹膜组,腹膜损伤组(无生物膜覆盖处理),NAC壳聚糖多孔膜组,壳聚糖多孔膜组,每组40只。应用EMSA方法检测Sp1含量,Western blot检测NF-κB,Masson染色法检测Sp1和SP染色法检测NF-κB表达。结果腹膜损伤导致Sp1和NF-κB被激活,壳聚糖多孔膜和NAC壳聚糖多孔膜覆盖损伤的腹膜可以一定程度抑制Sp1和NF-κB的表达。在抑制Sp1和NF-κB表达方面,NAC壳聚糖多孔膜显著优于壳聚糖多孔膜。NAC壳聚糖膜组比壳聚糖膜组更显著减低粘连级别(P<0.05或P<0.01)。结论 NAC壳聚糖生物膜可显著下调核转录因子Sp1和NF-κB表达,并有效减轻腹膜粘连形成。

Objective To investigate the expression changes of the nuclear transcription factors Spl and NF- K B by NAC（N-acetylcysteine） chitosan porous membrane in the process of peritoneal adhesion of rats. Methods NAC chitosan porous membrane was used to interference the formation of peritoneal adhesion, the animal model of abdominal surgery was established, 160 rats were averagely divided into： normal, peritoneal damage, chitosan porous membrane and NAC chitosan porous membrane groups. Ele~：trophoresis mobility shift assay（EMSA）, SDS-PACE, Masson and SP methods were used to detect the content and expressions of Spl and NF- K B in peritoneal membrane. Results Peritoneal damage activated the expressions of Spl and NF-K B, chitosan porous membrane and NAC chitosan porous membrane downregulated the expression levels of Spl and NF- K B, but stronger for NAC chitosan porous membrane than for chitosan porous membrane. And also the downregulation of the peritoneal adhesion grade was much more in NAC chitosan porous membrane than in chitosan porous membrane（P〈0.05）. Conclusion NAC chitosan porous membrane can downregulate the expressions of nuclear transcription factor Sp1 and NF- K B and effectively decrease the peritoneal adhesion formation.