氧糖剥夺诱导原代大鼠星形胶质细胞miR-21的表达变化

Alteration of miR-21 expression in primary rat astrocytes after oxygen-glucose deprivation

目的研究原代大鼠星形胶质细胞氧糖剥夺不同时间miR-21的表达,探讨miR-21是否参与了缺氧缺血性脑损伤后星形胶质细胞的活性变化。方法原代大鼠星形胶质细胞随机分为正常组和氧糖剥夺组,分别培养3、6、9和12h。CCK-8法检测各组星形胶质细胞的活力,real-time PCR方法检测各组星形胶质细胞miR-21的表达。结果氧糖剥夺组miR-21的表达水平随培养时间延长呈先升高后降低的动态变化,3h开始高于对照组水平(P<0.05),6h达高峰(P<0.05),随后下降,9h与对照组无差异(P>0.05),12h显著低于对照组(P<0.05)。氧糖剥夺不同时间星形胶质细胞活力变化与miR-21的变化趋势一致。结论 miR-21可能参与了缺氧缺血性脑损伤后星形胶质细胞的活性变化,发挥促增殖和抗凋亡的作用。

Objective To study the expression of miR-21 in primary rat astrocytes after oxygen-glucose deprivation（OGD） for different time, and to investigate whether miR-21 participates in the alteration of astrocytes after hypoxic ischemic brain injury. Methods Primary rat astroeytes were divided into the normal culture group and OGD culture group randomly, cultured for 3, 6, 9 and 12 hours respectively. The activities of primary astrocytes were measured by CCK- 8Kit staining. The expression of miR-21 was detected by real-time PCR. Results The expression level ofmiR-21 in OGD culture groups first increased and then decreased with prolonged culture time, miR-21 expression level began to increase in OGD culture groups（cultured for 3-hour）than in the control group（P〈0.05） and reached the peak in OGD（cultured for 6h, P〈0.05）, and then decreased near to the level of the eontrol group in OGD （cultured for 9h,P〉0.05）, reduced to be lower in OGD （cultured for 12h）than in the control group（P〈0.05）. The dynamic change of astroeyte activity in different OGD culture groups showed a consisent tendency with the alteration of miR-21. Conclusion miR-21 possibly participates in the activatiom of astroeytes after hypoxic-ischemia brain injury, and maybe promotes astroeyte proliferation and inhibits astrocyte apoptosis.