槲皮素对结肠癌LOVO细胞增殖侵袭能力及癌胚抗原CEA表达的影响

Effect of Quercetin on proliferation and invasion of colon carcinoma LOVO cells and the expression level of carcinoembryonic antigen

目的:研究槲皮素对结肠癌LOVO细胞增殖侵袭能力及癌胚抗原(CEA)表达的影响。方法:采用MTT法检测不同浓度的槲皮素对LO-VO细胞增殖能力的影响;内皮细胞粘附实验和小室侵袭实验检测槲皮素对LOVO细胞侵袭能力的影响;细胞免疫荧光、WB及RT-PCR检测槲皮素对LOVO细胞癌胚抗原CEA表达的影响。结果:槲皮素能显著抑制LOVO细胞的增殖,呈浓度剂量依赖关系,IC50约为40μmol/L;增强LOVO细胞与内皮细胞间的粘附能力,减弱其侵袭能力;并能显著抑制CEA蛋白及mRNA的表达水平,且呈浓度剂量依赖关系。结论:槲皮素对LOVO细胞的增殖侵袭能力具有抑制作用,并能抑制CEA的表达。

AIM： To investigate the effect of Quercetin on proliferation and invasion of colon carcinoma LOVO cells as well as the expression level of carcinoembryonic antigen. METHODS： LOVO ceils were treated with Quercetin at various concentrations, then determined for proliferation level by MTT assay, for cell adhesion and invasion of LOVO cell by tumor cell adhere to the vascular endothelial cell assay and transwell chamber invasion assay, and for the expression level of CEA by Immunocellulerchemistry （ICC）, Western Blot and RT-PCR. RESULTS： Significantly, Quercetin showed dose-dependent inhibitory effect on proliferation of LOVO cells with an IC50 of about 40μmol/L, also improved the ability of LOVO cells adhere to the vascular endothelial cells and reduced the invasion ability of LOVO cells. Meanwhile, Quercetin significantly inhibited the protein and mRNA expression levels of CEA in LOVO cells. CONCLUSION： Quercetin has inhibition effect on the proliferation invasion ability of LOVO cells,also inhibit the protein and mRNA expression levels of CEA in LOVO cells.