番茄WRKY转录因子in silico鉴定及表达分析

In silico Identification and Expression Analysis of WRKY Transcription Factors in Tomato

WRKY蛋白是植物中最大的转录因子家族之一,对植物的生长发育具有重要调控作用。本研究利用番茄全基因组测序结果鉴定了WRKY基因,分析了其系统发育关系,内含子-外显子结构,染色体上的分布及其表达方式。研究表明:番茄中存在81个WRKY转录因子,分为三类(Ⅰ,Ⅱ和Ⅲ),第Ⅰ和Ⅱ类分别细分为2和5个亚类,这些基因不均匀分布在番茄的11条染色体上;基因结构分析表明番茄WRKY转录因子进化过程中可能发生内含子的缺失/获得事件;不同芯片表达分析表明WRKY基因不仅参与了番茄根、子叶和真叶等不同组织类型的生长发育,而且还参与了一些生物胁迫(盐)和非生物胁迫(真菌激活子)的反应。

Proteins of WRKY family, one of the largest families of transcription factors, play an important role in regulation of plant growth and development. In this research, we investigated tomato WRKY family genes based on the result of the whole genome sequencing and analyzed their phylogenetic relationships, the structure of introns and exons, the distribution of the chromosome as well as the model of expression. The result showed that 81 WRKY ranscription factors exists in the tomoto genome, which can be divided three types （Ⅰ, Ⅱ and Ⅲ）, and Type Ⅰ, SlWRKY Ⅰ, can be subdivided 2 sub-classes and Type Ⅱ, SlWRKY Ⅱ can be subdivided 5 sub-classes; These genes unevenly distributes in 11 chromosomes of Tomoto. Analysis of the gene structure indicated that indel/acquire events of the intron may happen in the process of evolution of WRKY genes; Expression analysis of different chips revealed that SlWRKY not only involve in growth and development of different tissues such as root, cotyledon and leaf, but also response to biotic （salt） and abiotic （fungi activator） stresses.