瘦素及其受体在精索静脉曲张模型大鼠附睾组织的表达及意义

Increased expressions of leptin and its receptor in the epididymis of varicocele model rats and their implications

目的:探讨瘦素及其受体在精索静脉曲张模型大鼠附睾组织的表达及其意义。方法:将40只SD大鼠随机分为精索静脉曲张(EV)持续4、8周组(EV4、EV8组,每组12只)和相应的假手术对照组(Control 4、Control 8组,每组8只)。采用左肾静脉部分结扎的方法建立精索静脉曲张模型。采用免疫组织化学法检测大鼠附睾组织中瘦素及瘦素受体表达情况,采用实时定量PCR法检测大鼠附睾组织中瘦素及瘦素受体mRNA表达量。结果:EV4组、EV8组附睾上皮细胞瘦素及瘦素受体表达分别比Control 4组、Control 8组显著增强(P<0.01);而EV4组附睾上皮细胞瘦素及瘦素受体表达与EV8组比较无显著性差异(P>0.05)。EV4组、EV8组附睾上皮细胞瘦素及瘦素受体mRNA的表达分别比Control 4组、Control 8组显著增加(P<0.01);而EV4组瘦素及瘦素受体mRNA的表达与EV8组比较无显著性差异(P>0.05)。结论:精索静脉曲张大鼠附睾组织中瘦素及瘦素受体的表达明显增加,瘦素可能参与了精索静脉曲张引起男性不育的机制。

Objective： To investigate the expressions of leptin and its receptor in the epididymis of experimental varicocele（EV） rats.Methods： Forty male Sprague-Dawley rats were randomly divided into four groups： 4-week EV（n=12）,8-week EV（n=12）,4-week control（n=8）,and 8-week control（n=8）.EV models were established by partial ligation of the left renal vein.The expressions of leptin and its receptor in the rat epididymis were measured by immunohistochemistry,and their mRNA expressions determined by real-time quantitative PCR.Results： The expressions of leptin and its receptor in the epididymis were significantly higher in the 4-and 8-week EV groups than in the 4-and 8-week control groups（P0.01）,with no significant difference between the two EV groups（P0.05）.So were their mRNA expressions in the former two than in the latter two groups（P0.01）,with no significant difference between the former two（P0.05）.Conclusion： The expressions of leptin and its receptor are markedly increased in the epididymis of varicocele rats.Leptin may be involved in the mechanisms of varicocele inducing male infertility.