选择反应监测技术在蛋白质组学研究中的应用

Application of Selected Reaction Monitoring in Proteomics Research

基于三重四极杆质谱仪的选择反应监测(SRM)技术是一种根据已有信息或理论信息靶向进行质谱信号采集的技术,具有高选择性、高重复性、高灵敏度、宽动态范围等特点,已被广泛应用于蛋白质组学研究,用于生物样本中蛋白质的绝对定量分析.本文对SRM技术的特点、发展过程、在蛋白质组学中的应用现状以及发展前景进行了概述.

Selected reaction monitoring(SRM) is a technology performed on triple quadrupole mass spectrometers(MS),which aims to acquire targeted MS signals according to the given or predicted information.With high selectivity,repeatability,sensitivity and wide dynamic range,SRM has been widely applied to proteome research and used for protein absolute quantification in biological samples.This review mainly focuses on characteristics and development of SRM technology,as well as its application and future prospects in proteomics.