乳腺癌组织中CKS1和CyclinD1的表达及相关分析

Expressions and significances of CKS1 and CyclinD1 in breast carcinoma

目的:探讨细胞周期蛋白依赖性激酶调节亚基1(cyclin kinase subunit1,CKS1)和细胞周期蛋白D1(CyclinD1)在乳腺癌组织的表达及意义。方法:采用免疫组化SP法检测105例乳腺癌组织中CKS1和CyclinD1的表达,并同时检测100例乳腺纤维腺瘤作为对照,分析其在乳腺癌组织中的表达及临床意义。结果:CKS1和CyclinD1在乳腺癌组织中的阳性率分别为80.9%(85/105)和85.7%(90/105),在乳腺纤维腺瘤中的阳性率分别为22%(22/100)和19%(19/100)。乳腺癌中两基因蛋白的阳性表达与淋巴管侵犯、淋巴结转移有关;乳腺癌中两基因蛋白的阳性表达均明显高于乳腺纤维腺瘤(P<0.05);乳腺癌组织中CKS1与CyclinD1的阳性表达呈正相关(r=0.677,P<0.05)。结论:CKS1和CyclinD1在乳腺癌组织中的表达较对照组乳腺纤维腺瘤明显升高,并且与淋巴管侵犯、淋巴结转移呈正相关,提示检测2种蛋白的表达对乳腺癌的临床治疗及预后预测具有一定的意义。

Objective：To investigate the expressions and significances of cyclin kinase subunit1（CKS1）and CyclinD1 in breast carcinoma.Methods ：Expressions of CKS1 and CyclinD1 in 105 cases of breast carcinoma were assessed by immunohistochemical technique（SP method）and those in 100 cases of breast fibroadenoma（control group）were simultaneously detected.Expressions and significances of CKS1 and CyclinD1 in breast carcinoma tissues were analyzed.Results：Positive rates of CKS1 and CyclinD1 in breast carcinoma were 80.9%（85/105）and 85.7%（90/105）.Positive rates of CKS1 and CyclinD1 in breast fibroadenoma were 22%（22/ 100）and 19%（19/100）.Positive expressions of two genes were in correlation with lymphatic vessel invasion and lymph node metastasis in breast carcinoma.Positive rates of CKS1 and CyclinD1 in breast carcinoma were higher than those in breast fibroadenoma（P〈0.05）.Positive expressions of CKS1 and CyclinD1 in breast carcinoma were in positive correlation（r=0.677,P〈0.05）.Conclusions： Positive expressions of CKS1 and CyclinD1 are significantly higher in breast carcinoma than in breast fibroadenoma.Positive expressions of CKS1 and CyclinD1 in breast carcinoma are positively related with lymphatic vessel invasion and metastasis of lymph node.Expressions of CKS1 and CyclinD1 may play a role in evaluating the prognosis and guiding the clinical treatment for breast carcinoma.