海藻酸钙水凝胶微球悬浮液与全血的流变学性质比较

Comparison of Rheologic Properties between Ca-alginate Hydrogel Microspheres Suspension and Whole Blood

本文从红细胞形态以及红细胞压积(Hct约为45vol%)的角度出发,制备一种微球悬浮液来模拟全血的非牛顿流体性质,探索其在血液黏度质控物中的应用价值。我们采用乳液聚合法制备海藻酸钙水凝胶微球,并将其悬浮于0.9wt%的氯化钠溶液中,得到微球压积为45vol%的悬浮液样品。然后使用两种不同原理的黏度仪检测分析样品在不同切变率下的黏度变化。经显微镜观察,海藻酸钙水凝胶微球形态完整,直径近似服从正态分布,约90%的微球直径分布在6～22μm之间。用FASCO-3010型和LG-R-80c型血液黏度仪对同一样品进行检测,均显示出剪切稀化的性质。对样品进行连续5周的稳定性实验,两种仪器测得的对应样品黏度值变异系数分别为7.3%～13.8%和8.9%～14.2%。尽管样品在同一切变率下对应的结果之间存在一定差异,但不同切变率下结果的总体变化趋势是一致的,具备普遍适用于不同原理血液黏度仪校准的潜力。

Starting from the form of red blood cells and the hematocrit （Hct, about 45 vol% of whole blood）, we tried to prepare a kind of microspheres suspension to imitate non-Newtonian fluid property of whole blood, exploring its potentiality to be applied in blood viscosity quality control substance. In our study, we produced Ca-alginate hydrogel microspheres using emulsion polymerization, then we suspended the microspheres in 0.9 wt% NaC1 solution to ob- tain a kind of liquid sample with the microspheres taking 45 % volume. Then we used two types of viscometers to measure and analyse the changes of sample viscosity at different shear rate. We observed the forms of Ca-alginate hy- drogel microspheres with microscope, and found them to be relatively complete, and their diameters to be normally distributed. Diameters of about 90% of the microspheres were distributed in a range from 6 to 22 micron. The sampies were examined with viscometer FASCO-3010 and LG-R-80c respectively, both of which have shown a shear- thinning effect. After 5-week stability test, the CV of viscosity results corresponding to the two instruments were 7.3%to 13. 8% and 8.9% to 14. 2%, respectively. Although some differences existed among the results under the same shear rate, the general variation trends of the corresponding results were consistent, so the sample had the po- tentiality to be widely used in calibrating a different type of blood viscometer.