IL-6对食管癌细胞侵袭迁移及上皮间质转化的影响

Effects of interleukin-6 on invasion,migration and epithelial-mesenchymal transition in human esophageal carcinoma cells

目的研究白细胞介素-6(interleukin-6,IL-6)对食管癌Eca-109细胞增殖、侵袭迁移及上皮间质转化的影响,探讨其作用机制。方法用不同浓度(0、25、50和100 ng/mL)的IL-6处理Eca-109细胞,采用MTT法检测细胞增殖,细胞划痕实验、Transwell小室实验检测细胞迁移侵袭能力,荧光定量PCR检测E-cadherin、N-cadherin、Vimentin和Twist1mRNA的表达,Western blot检测Stat3、p-Stat3、E-cadherin、N-cadherin、Vimentin和Twist1蛋白表达。结果与对照组(0 ng/mL)比较,IL-6处理组(25、50、100 ng/mL)在IL-6作用后第1～5天Eca-109细胞增殖增加,呈量效-时效关系(P<0.05,P<0.01)。IL-6各浓度(0、25、50 ng/mL)组24 h细胞迁移距离分别为(18.20±0.89)、(23.77±0.40)和(25.27±0.93)mm,与对照组(0 ng/mL)比较,IL-6处理组(25、50 ng/mL)Eca-109细胞体外迁移距离明显增加(P<0.05,P<0.05)。IL-6各浓度(0、25、50 ng/mL)组穿过Matrigel胶的细胞分别为(70.33±2.36)、(103.00±3.51)、(118.00±4.00)个/视野,与对照组(0 ng/mL)比较,IL-6处理组(25、50 ng/mL)Eca-109细胞体外穿过Matrigel胶的细胞数量明显增加(P<0.05,P<0.05)。荧光定量PCR结果显示,与对照组(0 ng/mL)相比,IL-6处理(25、50 ng/mL)组N-cadherin、Vimentin和Twist1 mRNA达增加(P<0.05,P<0.05),E-cadherin mRNA表达降低(P<0.05)。Western blot结果显示,与对照组(0 ng/mL)相比,IL-6各处理(25、50 ng/mL)组p-Stat3、N-cadherin、Vimentin和Twist1蛋白表达增加,E-cadherin蛋白表达降低,Stat3蛋白表达没有明显改变。结论 IL-6可提高食管癌Eca-109细胞体外增殖、侵袭、迁移能力,促进上皮间质转化过程,其机制可能通过活化Stat3蛋白,上调Twist1的表达,进一步调控EMT蛋白(E-cadherin、N-cadherin和Vimentin)的表达。

Objective To determine the effects of interleukin-6 （IL-6） on the cell proliferation, inva- sion, migration and epithelial-mesenehymal transition in human esophageal carcinoma cells. Methods The human esophageal carcinoma Eta-109 cells were treated with IL-6 at a dose of 0, 25, 50 and 100 ng/mL, cell proliferation, migration and invasion were detected by MTT assay, cell scratch test and Transwell chamber assay respectively. The mRNA expression of E-eadherin, N-eadherin, Vimentin and Twistl were evaluated by real- time quantitative PCR. The protein expression levels of Stat3, p-Stat3, E-cadherin, N-cadherin, Vimentin and Twistl were detected by Western blotting. Results Compared with control group （0 ng/mL IL-6 treatment） , the cell proliferation was in a dose- and time-dependent manner when the Eca-109 cells were treated by IL-6 at 25, 50 and 100 ng/mL for 1 to 5 d （P 〈0.05, 0.01）. IL-6 at 0, 25 and 50 ng/mL resulted in cell 24-hour migration distance of 18.20 ±0.89, 23.77 ±0.40 and 25.27 ±0.93 mm respectively, with the 2 later doses significantly longer than the former （ P 〈 0. 05 ）. IL-6 of above doses also resulted in the number of 24-hour Matrigel-permeating Eea-109 cells of 70.33 ± 2.36, 103.00 ± 3.51 and 118.00 ±4.00/HP, respectively,with the number in the 2 later groups significantly larger than in the former group （P 〈 0. 05 ）. Real time RT-PCR showed that the mRNA expression levels of N-cadherin, Vimentin and Twistl were significantly increased and the mRNA expression level of E-cadherin was decreased in IL-6 treatment groups （25 and 50 ng/mL） , significantly different from those in 0 ng/mL group （P 〈 0. 05）. Western blotting showed that the protein levels of p-Stat3, N-cadherin, Vimentin and Twistl were significantly increased, and that of E-cadherin was decreased, while that of Stat3 had no change in IL-6 treatment groups （25 and 50 ng/mL） when compared with 0 ng/mL group （P 〈 0.05 ）. Conclusion IL-6 promotes the cell proliferation, invasion, migration and epithelial mesenchymal transition in human esophageal carcinoma cells by constitutively activating Stat3, up- regulating Twistl, and regulating EMT proteins （E-cadherin, N-cadherin and Vimentin）.