HPV病毒负荷量与血清VEGF预测宫颈癌新辅助化疗效果的价值探讨

The predictive value of neoadjuvant chemotherapy effect in cervical patients by human papillomavirus infection load and serum VEGF

目的:研究宫颈癌患者人乳头状瘤病毒(HPV)感染负荷量及血清血管内皮生长因子(VEGF)水平与新辅助化疗效果的相关性,探讨其预测宫颈癌化疗效果的可能性。方法:收集2010年6月至2011年12月云南省肿瘤医院宫颈癌患者40例,均接受新辅助化疗1～3个疗程,化疗结束后3～4周评价治疗效果。化疗前采用二代杂交捕获技术(HC-Ⅱ)检测HPV DNA负荷量,酶联免疫法(ELISA)检测宫颈癌患者血清VEGF水平,分析化疗效果与化疗前HPV负荷量、血清VEGF水平的关系。结果:(1)新辅助化疗临床有效率为85%;(2)宫颈癌患者HPV感染负荷量、血清VEGF水平与新辅助化疗效果呈负相关(P<0.05);(3)HPV感染负荷量与血清VEGF水平呈正相关(R=0.525,P<0.05);(4)HPV病毒负荷量、血清VEGF水平与宫颈癌患者临床病理类型、FIGO分期均无相关性(P>0.05)。结论:宫颈癌新辅助化疗效果明显,联合检测宫颈癌患者HPV病毒负荷量及血清VEGF可以有效预测宫颈癌新辅助化疗效果,该法简单、方便,值得临床推广应用。

Objective：To investigate the relationship between the human papillomavirus infection load,serum VEGF and neoadjuvant chemotherapy effect in cervical cancer patients and explore the possibility of their predictive value on the chemotherapeutic effect.Methods：40 cervical cancer patients in Yunnan Province Cancer Hospital from June 2010 to Dec.2011 were treated with 1 to 3 cycles of neoadjuvant chemotherapy and evaluated the therapeutic effects 3~4 weeks later after treatment completed.HPV infection load and serum VEGF level were detected by the second-generation hybrid capture（HC-Ⅱ HPV DNA） and ELISA.The relationship between the HPV load,serum VEGF and NACT effect was determined.Results：（1）The effective rate of NACT was 85%.（2）There were negative correlation between the HPV load,serum VEGF level and NACT effect（P0.05）.（3）There were positive correlation between the HPV load and serum VEGF level（R=0.525,P0.05）.（4）There were no significant correlation between the HPV load,serum VEGF level and pathological type,FIGO stage respectively（P0.05）.Conclusion：NACT has obvious antitumor effect to cervical cancer.HPV load and serum VEGF can predict cervical cancer NACT effect.As these methods are simple and convenient,clinical application are recomended.