盐度胁迫对大海马(Hippocampus kuda)幼体生长、组分及酶活力的影响

EFFECT OF SALINITY STRESS ON THE GROWTH,BODY COMPOSITION AND ENZYME ACTIVITIES OF JUVENILE HIPPOCAMPUS KUDA

在盐度为25的条件下,以大海马幼体为实验材料,通过设置不同的盐度胁迫组(盐度从25胁迫至5、15和35)的方法,对其生长、生化组分以及酶活力的影响进行了研究。结果表明:15盐度组大海马幼体的体重、生化组分、能值与对照组(盐度25)相比差异不显著(P>0.05),体长、成活率指标则显著高于对照组(P<0.05),然而5、35盐度组的生长指标、成活率、生化组分等则显著低于对照组(P<0.05)。15盐度组的SOD、CAT酶活性低于对照组水平(P<0.05),MDA的含量变化不显著(P>0.05);而5、35盐度组SOD、CAT和MDA含量与对照组相比,随着时间的延长,呈现逐渐升高的趋势(P<0.05);随着盐度的升高,AKP酶活性具有逐渐升高的趋势,而ACP酶活性则呈现降低趋势。

This article investigated effects of variable salinity stress （salinity 25 dropped rapidly to 5, 15, 35） on the growth, biochemical composition and enzyme activities in juvenile Hippocampus kuda. The results showed that weight, biochemical composition and energy value of H. kuda juvenile of 15 salinity group had no significant difference with the control group（salinity 25） （P〉0.05）, the indexes of growth and survival rates were significantly higher than those of the control group （P〈0.05）, however, the growth rate, survival rate and biochemical composition of the 5, 35 salinity groups were apparently lower than those of the control group （P〈0.05）, besides the SOD, CAT enzyme activities in 15 salinity group were lower than in the control group level （P〈0.05）, the changes of MDA content had no significance （P〉0.05）. The SOD, CAT and MDA contents of the 5, 35 salinity groups compared with the control group, which show rising trend with time elapse （P〈0.05）. With the increases of salinity, the AKP activity has a rising trend, and opposite of the ACP activity.