反胶团体系中酶催化蔗糖水解反应动力学研究

Research on Dynamics Enzyme Catalysis Sucrose Hydrolysis in Reverse Micelle System

反胶团体系是由表面活性剂和有机溶剂形成的非水体系。在由正戊醇 十六烷基三甲基溴化铵 (CTAB) 异辛烷组成的反胶团体系中 ,对酶催化蔗糖水解反应动力学进行了初步研究 ,制作了反应进程曲线 ,测定了最佳pH条件、酶浓度对反应速度的影响以及米氏常数 ,结果符合米氏理论 。

The reverse micelle system is formatted by surface active agent and organic solvent. The studies of enzyme catalysis sucrose hydrolysis reaction in reverse micelle system of n-pentanol-CTAB-p-octane is made.The pH optimal value,influence of reaction rate with concentration of enzyme and constant of Michaelis-Menten is measured.