青葙子与反枝苋子提取物对小鼠急性肝损伤保护作用的比较

Comparison of the protective effect between the seeds of Celosia argentea and Amavanthus retroflexus on CCl4-induced acute liver injury in rats

目的比较青葙子与反枝苋子提取物对CCl4所致小鼠急性肝损伤的保护作用,为反枝苋子能否替代青葙子以及其在临床上的有效应用提供科学依据。方法采用CCl4诱导小鼠急性肝损伤模型,将108只小鼠随机分成9组,即空白组、模型组、联苯双酯阳性对照组和青葙子与反枝苋子提取物高、中、低剂量组;以小鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)的含量及肝脏指数为指标,观察两种提取物对小鼠CCl4肝损伤的保护作用。结果青葙子提取物各剂量组可不同程度地抑制肝脏指数的升高,降低肝损伤小鼠血清中ALT和AST含量,提高血清SOD活性,尤其以高、中剂量组较为明显,有一定的量效关系。而反枝苋子仅高剂量组与模型组比较差异有统计学意义(P<0.05)。结论青葙子提取物对急性肝损伤小鼠的保护作用明显强于反枝苋子,在临床上用反枝苋子替代青葙子作为清肝明目药可能会影响其疗效,两种药材的混用需谨慎。

Objective To investigate the protective effect between the seeds of Celosia argentea and Amavanthus retroflexus on CCl4-induced acute hepatic injury in rats.Methods 108 rats were randomly divided into nine groups,including the blank group,the model group,the positive group,and two drug groups with the high,middle and low dose,respectively.The experimental model of hepatic injury was induced by CCl4 in rats.The contents of ALT,AST and SOD in serum and liver index were determined.Results Compared with the model group,the contents of both ALT and AST decreased,the activity of SOD improved and the liver index decreased in three different doses of the ethanol extract of the seeds of Celosia argentea,especially in the high and middle dose groups.But only the high dose group of the ethanol extract of Amavanthus retroflexus showed a significant difference when compared to the model group（P〈0.05）.Conculusion The ethanol extract of the seeds of Celosia argentea exhibits protective effects on liver injury induced by CCl4 in rats,which is remarkably stronger than that of the seeds of Amavanthus retroflexus.Thereby,the therapeutic effect might be affected when using the seeds of Amavanthus retroflexus instead of the seeds of Celosia argentea.