中华鳖温和气单胞菌病的病原研究

Studies on the pathogens of disease of Aeromonas sobria in Trionyx Sinensis.

从表现为背腹甲及四肢出现疖疮、裙边糜烂的患病鳖的心血、肝脏及腹腔液中分离到 NL、Nb1和Nb2 三株细菌 .经菌体形态特征、培养特性和生理生化特性测定 ,均鉴定为温和气单胞菌 ( Aeromonassobria) .小白鼠感染试验显示三株菌均有毒力 ,用 9× 10 8CFU/m LNL 菌株进行人工感染 30～ 4 0 g健康鳖 ,发病率为 10 0 % .从感染鳖的肝脏中分离到与原分离株形态及生理生化特性相同的菌株 .用 NL 菌株制备的抗血清分别与 Nb1和 Nb2 菌株进行玻板凝集试验 ,能发生明显的凝集反应 .药敏试验结果表明 :三株细菌对菌必治、头孢氨噻肟。

Three strains of bacteria were isolated from the liver and blood of the diseased Trionyx Sinensis.Through cultivation and physio\|biochemical test,three strains are identified as belonging to Aeromonas sobria. White mouses infection tests show the three strains is pathogenic.Small soft shelled turtle of 30～40g body weight are injected with the bacteria of 9×10\+8CFU/mL concentration,mordity is 100%.The characterestics of these strains identified from the artifically infected soft\|shelled turtle are identified the primary isolate.The strains of N\-\{b1\} amd N\-\{b2\} strongly react to anti\|serum of N\-L isolate with slide agglutination test.Durg\|sensitivity tests show that the three strains are highly sensitive to Ceftriaxone,Cefotaxime and Ceftazidime.