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正交试验法优化大血藤中总皂苷的提取工艺

Optimization of the Extraction Technology of Total Saponin in Sargentodoxa Cuneata by Orthogonal Design
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摘要 目的:优化大血藤中总皂苷的提取工艺。方法:采用L_9(3~3)正交试验设计,以大血藤中总皂苷为指标,考察提取溶剂,温度,料液比三因素对大血藤提取效率的影响。结果:以70%乙醇,温度50℃,料液比为1:30为提取条件,提取总皂苷的产量最高。 Objective:To optimize the extraction technology of total saponin in Sargenlodoxa cuneata.Method:Orthogonal design L_9(3~3)was used to screen the optimal extraction technology.The extraction solvent,temperature and solvent/feed ratio were confirmed as the factors,and total saponin was determined by ultraviolet spectrophotometry.Result:The optima]extraction conditions of total saponin were as follows;70%ethanol,50℃and solvent/feed ratio of 1:30.Conclusion:The technology is reasonable and practicable, and can be used in the further research of Sargentodoxa cuneata.
出处 《中国药师》 CAS 2012年第12期1723-1725,共3页 China Pharmacist
关键词 大血藤 提取工艺 正交试验 紫外分光光度法 Sargentodoxa cuneata Extraction technology Orthogonal experiment Ultraviolet spectrophotometry
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